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41.
Deok-Gyu Kim Jae-Hwan Park Jae-Lip Kim Bong-Kwang Jung Sarah Jiyoun Jeon Hyemi Lim Mi Youn Lee Eun-Hee Shin Terry A. Klein Heung-Chul Kim Sung-Tae Chong Jin-Won Song Luck-Ju Baek Jong-Yil Chai 《The Korean journal of parasitology》2015,53(1):135-139
A total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including Apodemus agrarius (n = 1,400), Microtus fortis (167), Crocidura lasiura (91), Mus musculus (32), Myodes (= Eothenomys) regulus (9), Micromys minutus (6), and Tscherskia (= Cricetulus) triton (3), were live-trapped at US/Republic of Korea (ROK) military training sites near the demilitarized zone (DMZ) of Paju, Pocheon, and Yeoncheon, Gyeonggi Province from December 2004 to December 2009. Small mammals were examined for their intestinal nematodes by necropsy. A total of 1,617 rodents (100%) and 91 (100%) soricomorphs were infected with at least 1 nematode species, including Nippostrongylus brasiliensis, Heligmosomoides polygyrus, Syphacia obvelata, Heterakis spumosa, Protospirura muris, Capillaria spp., Trichuris muris, Rictularia affinis, and an unidentified species. N. brasiliensis was the most common species infecting small mammals (1,060; 62.1%) followed by H. polygyrus (617; 36.1%), S. obvelata (370; 21.7%), H. spumosa (314; 18.4%), P. muris (123; 7.2%), and Capillaria spp. (59; 3.5%). Low infection rates (0.1-0.8%) were observed for T. muris, R. affinis, and an unidentified species. The number of recovered worms was highest for N. brasiliensis (21,623 worms; mean 20.4 worms/infected specimen) followed by S. obvelata (9,235; 25.0 worms), H. polygyrus (4,122; 6.7 worms), and H. spumosa (1,160; 3.7 worms). A. agrarius demonstrated the highest prevalence for N. brasiliensis (70.9%), followed by M. minutus (50.0%), T. triton (33.3%), M. fortis (28.1%), M. musculus (15.6%), C. lasiura (13.2%), and M. regulus (0%). This is the first report of nematode infections in small mammals captured near the DMZ in ROK. 相似文献
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Hyuk Ko Eun-Hang Lee Byoung-Jin Jeon Hye-Jin Choi Min-Jung Kang Jae-Chul Pyun 《Biotechnology and Bioprocess Engineering》2011,16(6):1248-1253
An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of
rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized
on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test
sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional
enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using
inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have
a high statistical correlation to the conventional ELISA method. 相似文献
46.
Web Tools for Rice Transcriptome Analyses 总被引:1,自引:0,他引:1
Gene expression databases provide profiling data for the expression of thousands of genes to researchers worldwide. Oligonucleotide
microarray technology is a useful tool that has been employed to produce gene expression profiles in most species. In rice,
there are five genome-wide DNA microarray platforms: NSF 45K, BGI/Yale 60K, Affymetrix, Agilent Rice 44K, and NimbleGen 390K.
Presently, more than 1,700 hybridizations of microarray gene expression data are available from public microarray depositing
databases such as NCBI gene expression omnibus and Arrayexpress at EBI. More processing or reformatting of public gene expression
data is required for further applications or analyses. Web-based databases for expression meta-analyses are useful for guiding
researchers in designing relevant research schemes. In this review, we summarize various databases for expression meta-analyses
of rice genes and web tools for further applications, such as the development of co-expression network or functional gene
network. 相似文献
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Characterization of an alternative splicing by a NAGNAG splice acceptor site in the porcine KIT gene
Jae-Hwan Kim Tao Zhong In-Cheol Cho Hyun-Tae Lim Chae-Kyoung Yoo Byeong-Woo Kim Jun-Heon Lee Jin-Tae Jeon 《Genes & genomics.》2011,33(2):179-186
The KIT gene has been shown to have multiple functions in hematopoiesis, melanogenesis, and gametogenesis. In addition, mutations of this gene cause pigmentation disorders in humans and mice and are responsible for coat color differences in pigs. While characterizing polymorphisms in the porcine KIT gene, we detected alternative splicing (AS) of the NAGNAG splice acceptor site at the boundary of intron 4 and exon 5. This AS event generated the E and I isoforms, characterized by insertion or deletion, respectively, of CAG at the borders of coding sequence. AS patterns measured in tissue samples from two randomly selected animals did not identified any tissue-specific outcomes. Analysis of AS patterns using three breeds demonstrated that Landrace and Large White pigs expressed both the E and I isoforms. In contrast, a subset of specimens from Korean Native Pigs (KNP) yielded a single I isoform. Alignment of the sequence from several species revealed that the region between the branch point sequence (BPS) and 3′ acceptor site is conserved. However, it is appeared that the selection of either the proximal or distal splice site varied between species. To test the breed specificity the NAGNAG splice acceptor site, we constructed two lineages of minigenes from KNP and Landrace pigs harboring breed-specific mutations. The minigene splicing assay demonstrated that both types of minigenes expressed both the E and I isoforms in two host cell lines, and no differences were detected in the AS pattern between the two breeds. We conclude that the AS at the NAGNAG splice acceptor site on intron 4/exon 5 in the porcine KIT gene is the result of noise selection at the splice site by the splicing machinery. Therefore, this AS event in the porcine KIT gene is unlikely to have any relationship with the coat color variations of Landrace and KNP breeds. 相似文献
49.
Jae-Hoon HwangJeong-A Choi Reda A.I. Abou-Shanab Booki MinHocheol Song Yongje Kim Eung Seok LeeByong-Hun Jeon 《Bioresource technology》2011,102(2):1051-1058
Anaerobic fermentation for hydrogen (H2) production was studied in a two-stage fermentation system fed with different ripened fruit feedstocks (apple, pear, and grape). Among the feedstocks, ripened apple was the most efficient substrate for cumulative H2 production (4463.7 mL-H2 L−1-culture) with a maximum H2 yield (2.2 mol H2 mol−1 glucose) in the first stage at a hydraulic retention time (HRT) of 18 h. The additional cumulative biohydrogen (3337.4 mL-H2 L−1-culture) was produced in the second stage with the reused residual substrate from the first stage. The major byproducts in this study were butyrate, acetate, and ethanol, and butyrate was dominant among them in all test runs. During the two-stage system, the energy efficiency (H2 conversion) obtained from mixed ripened fruits (RF) increased from 4.6% (in the first stage) to 15.5% (in the second stage), which indicated the energy efficiency can be improved by combined hydrogen production process. The RF could be used as substrates for biohydrogen fermentation in a two-stage (dark/dark) fermentation system. 相似文献
50.
Jin Goo Lee Jae‐Ha Myung Aaron B. Naden Ok Sung Jeon Yong Gun Shul John T. S. Irvine 《Liver Transplantation》2020,10(10)
For efficient catalysis and electrocatalysis well‐designed, high‐surface‐area support architectures covered with highly dispersed metal nanoparticles with good catalyst‐support interactions are required. In situ grown Ni nanoparticles on perovskites have been recently reported to enhance catalytic activities in high‐temperature systems such as solid oxide cells (SOCs). However, the micrometer‐scale primary particles prepared by conventional solid‐state reactions have limited surface area and tend to retain much of the active catalytic element within the bulk, limiting efficacy of such exsolution processes in low‐temperature systems. Here, a new, highly efficient, solvothermal route is demonstrated to exsolution from smaller scale primary particles. Furthermore, unlike previous reports of B‐site exsolution, it seems that the metal nanoparticles are exsolved from the A‐site of these perovskites. The catalysts show large active site areas and strong metal‐support interaction (SMSI), leading to ≈26% higher geometric activity (25 times higher mass activity with 1.4 V of Eon‐set) and stability for oxygen‐evolution reaction (OER) with only 0.72 µg base metal contents compared to typical 20 wt% Ni/C and even commercial 20 wt% Ir/C. The findings obtained here demonstrate the potential design and development of heterogeneous catalysts in various low‐temperature electrochemical systems including alkaline fuel cells and metal–air batteries. 相似文献